論文庫

論文題目 Cardiac Na+-Ca(2+)exchanger 1 (ncx1h) is critical for the ventricular cardiomyocyte formation via regulating the expression levels ofgata4andhand2in zebrafish
作者 Chu, Liming; Yin, Huimin; Gao, Lei; Gao, Li; Xia, Yu; Zhang, Chiyuan; Chen, Yi; Liu, Tingxi; Huang, Jijun; Boheler, Kenneth R.; Zhou, Yong; Yang, Huang-Tian
聯系作者 Zhou, Y; Yang, HT
發表年度 2021
刊物名稱 SCIENCE CHINA-LIFE SCIENCES
64
2
頁碼 255-268
摘要 Ca(2+)signaling is critical for heart development; however, the precise roles and regulatory pathways of Ca(2+)transport proteins in cardiogenesis remain largely unknown. Sodium-calcium exchanger 1 (Ncx1) is responsible for Ca(2+)efflux in cardiomyocytes. It is involved in cardiogenesis, while the mechanism is unclear. Here, using the forward genetic screening in zebrafish, we identified a novel mutation at a highly-conserved leucine residue inncx1gene (mutant(LDD353)/ncx1h(L154P)) that led to smaller hearts with reduced heart rate and weak contraction. Mechanistically, the number of ventricular but not atrial cardiomyocytes was reduced inncx1h(L154P)zebrafish. These defects were mimicked by knockdown or knockout ofncx1h. Moreover,ncx1h(L154P)had cytosolic and mitochondrial Ca(2+)overloading and Ca(2+)transient suppression in cardiomyocytes. Furthermore,ncx1h(L154P)andncx1hmorphants downregulated cardiac transcription factorshand2andgata4in the cardiac regions, while overexpression ofhand2andgata4partially rescued cardiac defects including the number of ventricular myocytes. These findings demonstrate an essential role of the novel 154th leucine residue in the maintenance of Ncx1 function in zebrafish, and reveal previous unrecognized critical roles of the 154th leucine residue and Ncx1 in the formation of ventricular cardiomyocytes by at least partially regulating the expression levels ofgata4andhand2.
學科 Biology
影響因子 4.611
論文類別 Article
国产激情无码一区二区三区|国产精品 私密保健会所|国产精品99久久久久久WWW|国产精品99久久久久久人
<蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>|